Clear S-to-P converted waves from the crust–mantle boundary (Moho) and lithosphere–asthenosphere boundary (LAB) have been observed on the eastern part of the Dead Sea Basin (DSB), and are used for the determination of the depth of the Moho and the LAB. A temporary network consisting of 18 seismic broad-band stations was operated in the DSB region as part of the DEad Sea Integrated REsearch project for 1.5 years beginning in September 2006. The obtained Moho depth (∼35 km) from S-to-P receiver functions agrees well with the results from P-to-S receiver functions and other geophysical data. The thickness of the lithosphere on the eastern part of the DSB is about 75 km. The results obtained here support and confirm previous studies, based on xenolith data, geodynamic modeling, heat flow observations, and S-to-P receiver functions. Therefore, the lithosphere on the eastern part of the DSB and along Wadi Araba has been thinned in the Late Cenozoic, following rifting and spreading of the Red Sea. The thinning of the lithosphere occurred without a concomitant change in the crustal thickness and thus an upwelling of the asthenosphere in the study area is invoked as the cause of the lithosphere thinning.